Working with DNA

Author: Stan Metzenberg
Publisher: Taylor & Francis
ISBN: 9780415374644
Format: PDF, ePub, Mobi
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This text gives advice on choosing the correct enzymes & vectors in order to achieve an appropriate product & discusses how to identify the product. It combines basic theory & simple explanations, contains protocols & advice on which protocol to use & why, & emphasizes interpretation of data & potential sources of error.

Measuring Gene Expression

Author: Matthew Avison
Publisher: Taylor & Francis
ISBN: 1134189176
Format: PDF
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In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.

Molecular Biology of the Cell

Author: Bruce Alberts
Publisher: Garland Science
ISBN: 1317563743
Format: PDF, ePub
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Human Molecular Genetics

Author: Tom Strachan
Publisher: Garland Science
ISBN: 1136844074
Format: PDF, ePub, Mobi
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Human Molecular Genetics is an established and class-proven textbook for upper-level undergraduates and graduate students which provides an authoritative and integrated approach to the molecular aspects of human genetics. While maintaining the hallmark features of previous editions, the Fourth Edition has been completely updated. It includes new Key Concepts at the beginning of each chapter and annotated further reading at the conclusion of each chapter, to help readers navigate the wealth of information in this subject. The text has been restructured so genomic technologies are integrated throughout, and next generation sequencing is included. Genetic testing, screening, approaches to therapy, personalized medicine, and disease models have been brought together in one section. Coverage of cell biology including stem cells and cell therapy, studying gene function and structure, comparative genomics, model organisms, noncoding RNAs and their functions, and epigenetics have all been expanded.

PCR

Author: MCPHERSON.
Publisher: Garland Science
ISBN: 1859960170
Format: PDF, Mobi
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This updated and revised edition provides both an introduction to PCR (polymerase chain reaction) for those new to the technique, and coverage of the wide range of current applications. PCR has revolutionized and underpins modern molcular biology. By focusing on current PCR technology, the book should be of interest to both first-time users and experienced researchers, as well as for the bioscience undergraduate.

Protein Purification

Author: Philip L.R. Bonner
Publisher: Garland Science
ISBN: 0203967267
Format: PDF, Docs
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Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.

Fundamentals of Biochemical Calculations Second Edition

Author: Krish Moorthy
Publisher: CRC Press
ISBN: 1420053582
Format: PDF, ePub, Docs
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Fundamentals of Biochemical Calculations, Second Edition demystifies the fundamental calculations used in modern biochemistry, cell biology, and allied biomedical sciences. The book encouragesbothundergraduates and scientists to develop an understanding of the processes involved in performing biochemical calculations, rather than rely on memorized formulae. Detailed calculation steps, useful comments, and mathematical hints enhance solved problems throughout... Encouraging the use of the Ratio Method, the author draws attention to its applicability to a wide variety of biochemistry problems with logical consistency and intuitive ease. This entirely revised and updated second edition introduces three new chapters with coverage of laboratory-oriented calculations and data presentation, calculations relating to the emerging techniques in DNA and molecular biology, and applying the Ratio Method to pharmaceutical calculations. New edition adds questions and extra tips in every chapter to help students perform biochemical calculations on their own! By strengthening their understanding of mathematical logic and problem solving skills, Fundamentals of Biochemical Calculations, Second Edition enables students and biochemists to explore a wider range of real-world applications with the ability to tackle increasingly complex problems in biochemistry and related fields.

The Basics of Self Balancing Processes

Author: Gordon Ghirann
Publisher: CRC Press
ISBN: 1466552581
Format: PDF, ePub
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Self-Balancing is not just a tweak or change to assembly line balancing, but a completely transformed method for achieving continuous flow. Among the reasons you should try Self-Balancing is that you can expect a productivity improvement of at least 30 percent—with improvements of 50-60 percent quite common. Using a well-tested method for successful improvements initiated by the author, The Basics of Self-Balancing Processes: True Lean Continuous Flow is the first book to explain how to achieve continuous flow in both simple and complex manufacturing environments. It describes how to recognize and resolve weak links to ensure continuous flow in your manufacturing operations. The book offers rules, tools, and guidelines to help you not only solve problems at the root, but even eliminate them before they start. It reviews the shortcomings of traditional assembly line balancing and walks readers through the new paradigm of Self-Balancing. The text includes a comprehensive overview that demonstrates the power, flexibility, and breakthroughs possible with this method. Offering solutions to the shortcomings associated with standard line balancing—including inventory buffers, variation, and operator pace—it provides you with the tools and understanding required to deal with batch and off-line processes, debug your line, arrange your parts and tools, and design your own Self-Balanced cells. Watch Gordon Ghirann discuss how his book can increase the productivity of your business. http://www.youtube.com/watch?v=yte0622XbcI&feature=youtu.be

Management Dilemmas

Author: Eli Schragenheim
Publisher: CRC Press
ISBN: 1498738095
Format: PDF, ePub, Docs
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An incredible ability awaits managers who practice Theory of Constraints (TOC) techniques: they can take a problem, look beyond the less important details, and directly identify the source of trouble. They've been known to promptly resolve perplexing matters - while the uninformed remain stuck. So many more managers could gain the benefit of TOC thinking... if they only took the time. Eli Schragenheim now offers an informative and enjoyable self-learning method, proving how TOC can be invaluable at a wide variety of workplaces. Management Dilemmas: The Theory of Constraints Approach to Problem Identification and Solutions conveys TOC methods through "virtual experience"-stories of managers and the situations they need to resolve. Take note of the dilemmas they're facing. Think about how you would respond under those circumstances. Then, compare your reactions with Schragenheim's TOC-influenced analysis. Associated with Dr. Eli Goldratt (the founder of TOC) for seven years, Schragenheim doesn't tell how the stories end. Instead, he encourages the reader to try out TOC techniques-especially the need to arrive at the most precise answer by raising the right questions. The conclusions you reach today could greatly help your on-the-job thinking tomorrow!